NADPH Oxidase Activity Is Independent of p47
نویسندگان
چکیده
منابع مشابه
Activation of the Phagocyte NADPH Oxidase Protein p47
Activation of phagocyte NADPH oxidase requires interaction between p47 and p22. p47 in resting phagocytes does not bind p22. Phosphorylation of serines in the p47 C terminus enables binding to the p22 C terminus by inducing a conformational change in p47 that unmasks the SH3A domain. We report that an arginine/lysine-rich region in the p47 C terminus binds the p47 SH3 domains expressed in tande...
متن کاملVascular Effects Following Homozygous Disruption of p47 An Essential Component of NADPH Oxidase
Background—Evidence suggests that the vessel wall contains an oxidase similar, if not identical, to phagocytic NADPH oxidase. We tested the contribution of this specific oxidase to the progression of atherosclerosis and the regulation of blood pressure. Methods and Results—An examination of aortic rings from wild-type mice and mice with homozygous targeted disruptions in p47 revealed that p47 k...
متن کاملVascular effects following homozygous disruption of p47(phox) : An essential component of NADPH oxidase.
BACKGROUND Evidence suggests that the vessel wall contains an oxidase similar, if not identical, to phagocytic NADPH oxidase. We tested the contribution of this specific oxidase to the progression of atherosclerosis and the regulation of blood pressure. METHODS AND RESULTS An examination of aortic rings from wild-type mice and mice with homozygous targeted disruptions in p47(phox) revealed th...
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In this report, we demonstrate that NADPH oxidase is activated by tumor necrosis factor-a (TNF-a) plus interferon-g (IFN-g) in human monocytic cells (THP-1 cells) differentiated with phorbol ester (PMA) and that physiological concentration of 17h-estradiol inhibits NADPH oxidase activity in THP-1 cells stimulated with TNF-a plus IFN-g. This effect is mediated by estrogen receptor based on estro...
متن کاملPhosphorylation of the leucocyte NADPH oxidase subunit p47(phox) by casein kinase 2: conformation-dependent phosphorylation and modulation of oxidase activity.
The leucocyte NADPH oxidase of neutrophils is a membrane-bound enzyme that catalyses the reduction of oxygen to O(-)(2) at the expense of NADPH. The enzyme is dormant in resting neutrophils but becomes active when the cells are exposed to the appropriate stimuli. During oxidase activation, the highly basic cytosolic oxidase component p47(phox) becomes phosphorylated on several serines and migra...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1996
ISSN: 0021-9258
DOI: 10.1074/jbc.271.37.22578